mouse and human hprt quantitect primer assays  (Qiagen)

Journal: Immunity

Article Title: Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation

doi: 10.1016/j.immuni.2017.11.020

Figure Lengend Snippet: LTi-like ILC3s Highly Express GPR183 and Migrate toward 7α,25-OHC (A) Gpr183 mRNA expression in the indicated cell populations from the spleen (n = 2–6). mRNA expression was normalized to Hprt . (B) GFP expression in lamina propria B cells and ILC subsets from the colon of Gpr183 GFP / + reporter mice (green histograms) and B6 control mice (gray histograms). (C) Left panel illustrates high GPR183-GFP expression in CD4 + LTi-like ILC3s from the colon. Right panel shows mean fluorescence intensity (MFI) of GPR183-GFP expression in the indicated cell populations from (B) (n = 6). (D–F) Transwell migration of splenic LTi-like ILC3s (Lin − CD90.2 + CD127 + NK1.1 − ) from Rag1 -deficient Gpr183 +/+ and Gpr183 −/− mice (D), splenic B cells from B6 mice and ILC subsets from Rorc(γt) GFP Rag1 −/− mice (E), and colonic ILC subsets from Rag1 −/− mice (F) to 7α,25-OHC (n = 2–3). Data are represented as means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 by one-way ANOVA with Tukey’s post-test. Data are representative of or combined from two (D and E) or three (A–C and F) experiments. See also and .

Article Snippet: Quantitect primer assay: Mouse Hprt , QIAGEN , 330001.

Techniques: Expressing, Control, Fluorescence, Migration

Journal: Immunity

Article Title: Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation

doi: 10.1016/j.immuni.2017.11.020

Figure Lengend Snippet: GPR183 Enhances IL-22 Production by Colonic ILC3s (A) IL-22 production by ILC3s from the colon or small intestine of Gpr183 +/+ and Gpr183 −/− mice. Numbers indicate cell frequencies. (B) Frequency of intestinal ILC3s producing IL-17A, IL-17F, and IL-22 and frequency of ILC2s producing IL-5 from Gpr183 +/+ and Gpr183 −/− mice (n = 3–4). (C) Frequency of IL-22-producing ILC3s from mesenteric lymph nodes (MLN), small intestine (SI), and colon of B6 mice after stimulation with IL-1β and IL-23 in the presence of solvent control dimethyl sulfoxide (DMSO) or 10 nM 7α,25-OHC (n = 10). (D) Csf2 mRNA expression in the colon (top; n = 8–9) or in sorted colonic ILC3s (bottom; n = 2) from Gpr183 +/+ and Gpr183 −/− mice on a Rag1 -deficient background. mRNA expression was normalized to Hprt . Data are represented as means ± SEM. ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s t test. Data are representative of or combined from two experiments. See also Figure S6 .

Article Snippet: Quantitect primer assay: Mouse Hprt , QIAGEN , 330001.

Techniques: Solvent, Control, Expressing

Journal: Immunity

Article Title: Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation

doi: 10.1016/j.immuni.2017.11.020

Figure Lengend Snippet:

Article Snippet: Quantitect primer assay: Mouse Hprt , QIAGEN , 330001.

Techniques: Control, In Vivo, Recombinant, Cell Stimulation, Reverse Transcription, cDNA Synthesis, Purification, Transgenic Assay, Quantitative RT-PCR, Software, TaqMan Assay